Chemistry



Q. What is a Chromatorgraphy?

Ans. Chromatography is the separation of mixtures of compounds. There are many uses for it (one of them quantitative analysis... how much of a compound is in a sample). Anyway, there are many types of chromatography... Gas, Liquid... for liquid, there is Affinity, ion-exchange, size-exclusion, and many others. The principle that this is based on is that different compounds are run through a column consisting of a stationary and a mobile phase... the rate at which each compound moves through the column is proportional to the amount of it that binds (reversibly) to the stationary phase as opposed to the amount that stays in the mobile phase. In HPLC (high performance liquid chromatography), this rate (the retention time) is different for each compound, so that very good resolution of sample components is achieved.



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